![]() "Regolith" is the industry term for loose mineral material covering bedrock.Įxcavators become more technically advanced each year. The Regolith Advanced Surface Systems Operations Robot (RASSOR) Excavator is a mini-mover that works in low-gravity environments such as those on the Moon, Mars, asteroids and comets. ![]() Some excavators are designed with the future in mind. The Cat 300.9D has a 13 horsepower engine running its tracks, bucket and blade. Don’t think that small size means low power, though. It can sneak through a doorway to take on interior demolition challenges. It measures 28 inches wide and has a retractable undercarriage. On the small-scale end, there's the Cat 300.9D. The machine is big enough to see in satellite photos. It has a bucket wheel feature that handles 8.5 million cubic feet per working day. It’s the Bagger 293 mining machine, which tips the scale at 14,200 tons. The world’s largest land vehicle title also goes to an excavator. Its twin 4,500 horsepower engines drive a 65-yard bucket that moves 9,000 tons of material per hour. The behemoth Bucyrus is 32 feet tall, 24 feet wide and weighs 980 tons. The world record for the largest excavator goes to the Bucyrus RH400. Over the years, some excavators have set world records. It wasn’t long before Cat engineers put tracks under their hydraulic excavators, revolutionizing the earthmoving world. Cat® machines were the first to use steel-linked tracks, which propelled their first bulldozers. The Caterpillar Tractor Company entered the heavy equipment scene in 1925. It was the first truly hydraulic excavator without chains or cables. By 1897, the Kilgore Machine Company introduced the Direct Acting Excavator. featured steam-driven cylinders moving cables. Hydraulic excavators, which also use steam power, date to 1882. A team of men with picks and shovels could only dig 12. It could move 300 yards of material per day. By the mid-1800s, his steam shovel invention was the backbone of railway construction. William Otis assembled a highly functional system of gears, chains, pulleys and cables to excavate dry earth. Mechanized excavators have been around for over two hundred years. If you're considering renting or using an excavator, get the full story on these dependable digging tools. Excavators also have complex parts and a surprising array of attachments available. You’ll find several the different types of excavators available, such as mini-excavators, backhoe excavators, extended-reach excavators and even excavators built to use suction rather than teeth. It’s easy to get confused when researching excavators. There’s no such thing as a one-size-fits-all application when it comes to excavators. It’s additional questions like what excavator types, what sizes, what power and what attachments you’ll find around excavation jobs. It’s not just a question of using an excavator to dig, load or spread. If there’s material to move, no doubt you’ll find an excavator nearby. ![]() They’re also invaluable for general construction and utility work, demolition and scrap businesses, quarry and aggregate operations as well as handling waste and recycling products. Excavators are indispensable equipment pieces in the earthmoving business.
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3/31/2024 0 Comments Best college planners with budget![]() The Hobonichi Techo is a longtime Strategist favorite, used by three of our present and former staffers (all three of whom have used the planner for years to stay organized). This long-term testing and devotion has earned it the top spot here. Layout: Daily | Size: 4 by 6 inches | Cover: Softcover Any of the 33 planners recommended below will surely go a long way toward keeping you on track this year. I’ve also included my own thoughts on our best overall planner, which I’ve been testing personally for the past five years. ![]() So to find the best planners, I talked to 32 productivity experts, life coaches, and people who love stationery (including a few Strategist staffers) about their favorites, then culled our archives for standout styles we’ve written about before. ![]() (I have, after all, updated this list faithfully every January and was tasked with selecting the best 100 notebooks for our massive notebook-testing story.) While I’ve been devoted to Hobonichi Techo for most of my adult life, I’ve covered the Strategist’s stationery beat long enough to know that finding the right planner out of the very vast and very particular world of paper goods can be overwhelming. Leafing through the well-loved Hobonichi Techo planners I’ve used over the years brings me almost as much pleasure as scribbling in them did. And once the calendar year is complete, a physical planner becomes an artifact - a time capsule, if you will - of its happenings. There is plenty of research that shows how actually writing down your to-do list and schedule for the day - rather than typing it out on a laptop or iPhone - makes you feel more engaged in the task at hand. Understanding what you can afford for college and creating your college budget allows you to plan for college confidently.Instead of an app or gadget that claims to jump-start your productivity, consider investing in a good paper planner. If you still have more to pay, it’s time to figure out how or if you want to cover that difference, or if you need to cross that school off the list. If you’ve paid for all of it- WAY TO GO! Your college budget work paid off. When you are armed with your newly figured college budget, you can look at the total cost of each college your child may be interested in, subtract discounts and merit the school will offer, then subtract how much you can pay and see if you have any remaining balance. If you have a high schooler, it’s time! Get your head around your college budget early so you can understand affordability. Grandparents (learn more here about grandparent 529 plans).Do you pay for sports or other types of extracurricular activities?ĭo you want your kid to have some “skin in the game” financially for their own education?ĭo you have people who want to help cover some of the cost of college for you kids? This is another bucket of money you need to think about as you are making your college budget.These expenses will more than likely go away when they go to college so could you allocate this money to the college budget each month now? Think about what you are currently spending on your child while they are living at home. ![]()
3/31/2024 0 Comments Smaart v8 demo![]() Smaart SPL includes all of the multi-channel, remotely monitorable, SPL metering and logging features found in Smaart Suite, in a simplified, purpose-focused package. ![]() Smaart SPL (SPL Edition) is the power of Smaart Suite’s SPL mode as a stand-alone edition. And because it uses the same GUI and command and control structure as RT and Suite, it serves as the perfect gateway to the Smaart measurement platform for new users, students, and those who just want quick, simple, solid measurement functionality without the bells and whistles. Although it does not include many of the advanced user functions included in Suite and RT, it does allow for Smaart’s unlimited multi-engine measurement configurations and contains the core, essential functionality for Smaart’s spectrum and frequency response measurements. All the power of RT without all of the variables. Smaart LE (Light Edition) is a simplified, streamlined version of Smaart's Real Time Mode with many of the advanced settings and adjustments for Spectrum, Transfer Function and Live IR measurements pre-set and fixed – in effect, just like operating RT on its default measurement settings. Smaart RT is designed for users who simply require the complete advanced functionality of the Real-Time Mode. While it does include Live IR measurement and basic single-channel SPL monitoring capabilities, Smaart RT does not include the Suite’s acoustic analysis focused Impulse Response Mode or the fully featured SPL monitoring tools of SPL Mode. ![]() ![]() If you hear an engineer talking about using “Smaart,” they are probably talking about the RT mode. It comprises all of the core multi-engine FFT-based measurement capabilities that professional audio engineers have come to rely upon from Smaart over the past 27 years – RTA, Spectrograph, Transfer Function and Live Impulse Response measurements - all harnessed in a work environment developed to fit the workflow and task-critical demands of modern audio engineering. Smaart RT (Real-Time Edition) is Smaart Suite's Real-Time Mode as a stand-alone edition. Smaart Suite is the analog to the comprehensive functionality currently found in Smaart v8 and is designed for professional users who require (or desire) Smaart's full feature set across all three measurement modes. Smaart v9 will include the full-featured, flagship, Smaart Suite edition, which includes all three of Smaart's powerful measurement modes (Real-Time, Impulse Response, and SPL) with no functional limitations. The only difference between the four editions is in each one's feature sets. Smaart v8, Smaart Di v2, Smaart SPL v1), there will now be only one version of Smaart (v9) that is available in several different editions.īy standardizing all editions of Smaart on the same v9 code base, we have ensured that all editions share the same fundamental GUI, the same command and control structure, the same operational paradigms, and are cohesive down to the hotkeys. This means that instead of maintaining multiple separate products, with separate but related code bases, each at different release versions (i.e. With the release of v9, Smaart is moving from its old version-based product structure to a new, more cohesive edition-based one. The Smaart brand went through a few ownership changes until Rational Acoustics was founded in 2008 with the specific purpose of ensuring the continued development of the Smaart platform while substantially enhancing the educational and support efforts that back it. Smaart was first developed in 1995 as a cost-effective software-based solution to audio analysis during a time when the choices for audio analyzers were limited to a small number of moderately to extremely expensive hardware devices – with very limited functionality by today’s standards. Once you’ve cleared all the fog in Tsushima’s northern territory, you can freely explore the entire area to find its collectible locations. Jogaku prefecture has seven places to liberate: General Bartu’s Camp, High Point Guard Post, Frozen Forest Crossroads, Silk Farm, Kawachi Whaling Village, General Dogshin’s Camp, and Mountain Pass. Jogaku Prefecture liberation locations map Image: Sucker Punch/Sony Interactive Entertainment via Polygon Sago prefecture has four places to liberate: White Falls Village, Bitter Hills Garrison, Frozen Overlook, and Derelict Mine. Sago Prefecture liberation locations map Image: Sucker Punch/Sony Interactive Entertainment via Polygon This portion of the Ghost of Tsushima guide includes all the Sashimono Banner Locations across Act 1: Izuhara, Act 2: Toyotama, and Act 3: Kamiagata. Kin has four locations to liberate: Twilight’s Edge Overlook, Morimae Brewery, Izumi Village, and Icy Forest Crossroads. Kin Prefecture liberation locations map Image: Sucker Punch/Sony Interactive Entertainment via Polygon Jogaku Prefecture Mongol-controlled liberation locations map Map of Izuhara, the southernmost region of Tsushima Island, with marked locations of Main / Mythic / Side Tales, Hidden Altars, Mongol Artifacts, Records, Singing Crickets, Hot Springs, Shinto Shrines, Fox Dens / Inari Shrines, Bamboo Strikes, Pilars of Honor, Mongol Territories, Haiku, and other points of interest.Sago Prefecture Mongol-controlled liberation locations map. ![]() Kin Prefecture Mongol-controlled liberation locations map. ![]() This page of our guide to Ghost of Tsushima has locations of all Haiku in the Kamiagata region. There aren’t any incomplete ones on my map. Ghost of Tsushima: Haiku, Kamiagata Region secrets map, video guide Ghost of Tsushima guide, walkthrough. I’ve completed every shrine, pillar, hot spring, etc. Image: Sucker Punch/Sony Interactive Entertainment via PolygonĬlick the links below to go directly to one of the prefectures below: Ghost of Tsushima messed around with the time stream a little bit and introduced haiku to Jin Sakai in the 13th century, allowing him to reflect on life’s themes during his journey through the. I’m working towards platinum so after liberating the whole island I went around to every question mark on the map (that I had left) and did each of them. Below we’ll detail the location of each occupied area by prefecture. There are 15 locations to liberate in Kamigata. Our Ghost of Tsushima map below will show you every location you need to liberate from Mongol control to clear out Kamiagata. However, if you liberate the island’s three main sections, you can uncover the entire map revealing every Undiscovered Location. If you’ve already liberated all of Izuhara and Toyotama, then you’ll get two Trophies for this area and the Master Liberator Trophy which you receive for liberating the entire island. Ghost of Tsushima’s titular island is big. Liberating all of Kamiagata will lift the fog of war surrounding the area, opening up the northern region to reveal a significant chunk of places to find like haiku locations, fox dens, and more. The final area of the game will still keep you plenty busy before and after you finish the campaign. ![]() Ghost of Tsushima’s northern region, Kamiagata, has 15 areas to liberate from Mongol control. 3/31/2024 0 Comments Mature cream pies![]() There’s not long to go for the countdown to the day the Adult Cream Pies become available at McDonald’s branches around Japan, as they go on sale for a limited time from Jan 15. ![]() Therefore, the name of the dessert won’t necessarily raise eyebrows amongst the majority of McDonald’s diners here in Japan, but for English speakers on Twitter, the Adult Cream Pie is causing a bit of a stir. While the word otona literally translates to “adult” in English, it should be noted that the adult entertainment industry and its related goods and products in Japan use the word adaruto, a corrupted form of the English word “adult”. The accompanying commercial for the new product also displays some subtext that allows for reading-in-between-the-lines. However, the combination of the word “adult” and “cream pie” brings up a whole other imagery entirely for English speakers, which has people questioning whether or not the team consulted with any English-language speakers before deciding on the new name. Otona is a word often used in the Japanese sweets industry to describe confectionery that has less sweetness than regular brands, evoking a sense of high-quality, refined flavor. Now they’re attempting to grab the spotlight once again with another never-before-seen release - the Otona no Cream Pie, which translates to “Adult Cream Pie” in English. ![]() In order to increase their popularity with fast food lovers around the nation, McDonald’s Japan has been working hard over the last five years, bringing out a slew of new burgers, collaborating with local brands and companies, and even opening a fancy pop-up restaurant with silverware, tablecloths, and wine glasses filled with iced tea. With so many local and international fast food chains in the country, McDonald’s Japan has a lot of competition to contend with, especially after numerous scandals in the first half of the last decade led to unprecedented deficits for the company. ![]() Celebrating Halloween at Disneyland is a must-do experience for young and old. The spookiest time of year at the happiest place on Earth is the period between September 7 and October 31, when the Disneyland Parks receive a haunting makeover (check dates as they change annually). The seasonal pets that must be crafted are listed below.A guide to celebrating Halloween at Disneyland California Octo/ Anaheim, California, Disney Travel, Disneyland, Travel, USA / 0 comment There are SEASONAL pets and ACHIEVEMENT pets….some seasonal pets are also achievement pets. Snow Griffin – free at level 7 (but an egg will sometimes show up on the Fortune Wheel)ĮTA: It should be noted that all of the Original Pets have eggs that need to be "hatched" - EXCEPT FOR the Winged Cat.he just requires coins and thank yous Pink Piggy – egg from Breadwinner, level 2 Hopping Tiger – egg from Dragon Fighter, level 2 Gold-Bearing Turtle – egg from Lucky One, level 3 If there is nothing next to the egg, then it is only by way of gift or Fortune Wheel.)įlap-Eared Puppy – egg from Steer Clear, level 2 (If an egg can be received from the achievements, it is so noted. There are SOME that are in the achievements. You can get the original eggs from the Fortune Wheel or gifts from friends. Look in the Laboratory under the paw (over the left column) to see what each requires for crafting. Except for the Winged Cat, they ALL have eggs. Original Pets – There are 19 original pets. Since this is a reference thread, please place comments and/or questions in the Comments thread provided in the Tips, Tricks, Guides, and Advice folder. ![]() Latest Feb-22 by datsalotta Howling with the HostessĢ0527 messages in 1311 discussions Oh the Absurdity! ![]() Latest Mar-5 by Trixie Belle (AnnaDownes01) Navalny killed 6 Latest Mar-5 by Lady Pirate (MCPirateLady) Having surgery 9 Latest Feb-18 by AEGram Messages from the HeartĢ145 messages in 131 discussions Lost my husband 21 Latest 5/23/23 by Crystal P (12earth45) Achievements and Musingsģ673 messages in 129 discussions Who has 10 million in coin? 777 Latest 10/27/18 by katiek2 The Consortiumģ65 messages in 14 discussions Consortium Directory ~Seek Help/Donate 300 Latest 5/24/22 by Moonki Master Name Listing - Android 5 Latest 9/23/22 by Cherowah Master Name Listing - IOS 5 Latest Mar-5 by Trixie Belle (AnnaDownes01) Seasonal Events, CCs and Updatesġ0347 messages in 1075 discussions Event Ghosts/Floaters - What they look l 16ġ055 messages in 19 discussions Master Name Listing - PC 6 Latest Mar-5 by Tammy27 (DoubleMsMom) Post your jokes here 1088 Latest Mar-5 by LvlSlgr Alpha Game 173-The Last Letter 36396 Latest Mar-5 by LvlSlgr Song Title Chain Game 20532 Latest Sep-21 by Tammy27 (DoubleMsMom) Fun and Gamesħ8878 messages in 21 discussions Game No. Latest 12/18/18 by AEGram COMPLETE INDEX - Tips, Tricks and Guides 3 Latest Feb-26 by LvlSlgr Tips, Tricks, Guides and AdviceĤ690 messages in 300 discussions PETS - How you get them 4 Latest Mar-3 by Kid (Kidmagnet) How Cold Is It? 200 Latest Mar-5 by ~J (amsavs) Grandma bragging rights 87 Latest Feb-20 by Robert (amf368) Fletcher's Tea Room and BarĤ019 messages in 82 discussions Books - what are you reading? 942 Latest Mar-1 by WasCin Transferring game from Old Kindle Fire to New 2 Latest Mar-1 by Lisadengel PROBLEM LOADING MY GAMES 4 Latest 9/2/23 by AEGram Titan's Hand in level 95 17 Latest Oct-10 by Sadie (Sadie700) Technical Issues FAQ Links 86 Latest Nov-20 by CHARLIE (charlieisr) Links for Game Support 16 Latest Jan-25 by AEGram Delphi's Friend and Private Message Features 63 Latest Feb-18 by Di (amina046) Technical IssuesĨ516 messages in 765 discussions No Update on PC-Try This to Fix 41 Latest Feb-9 by Rosa1949 Gifts, Wishes and Random Pleas for Helpġ072 messages in 117 discussions Ancestors Spirit 4 Latest Feb-12 by EndorWitchOf Friends of LISA - 910622 3 Latest Feb-17 by jamebo Frogger - Are You Out There? 2 Latest Feb-23 by WasCin “Deleted” Friends 24 Latest Feb-25 by Trixie Belle (AnnaDownes01) Friends in Invited list again! 1 Latest Feb-28 by misstracy22 Friends of Cattipuss and Silky games 5 Latest Feb-28 by Tammy27 (DoubleMsMom) To friends of games DotJ Ree DJ1 KelliJo 26 Latest Mar-2 by Tammy27 (DoubleMsMom) FRIENDS OF RANDY 34 Latest Mar-3 by lizziep13 Starting MC OVER 2 Latest Mar-4 by Robinj18 SHYLO returns with new ID 14 Latest Mar-4 by akge Had to reload game- need friends 9 Latest Mar-5 by WYOMARE Friends of Kae/Guinevere 1 Latest Jan-10 by DorceAn Looking for Gandalf 2 Latest Feb-13 by datsalotta Android Friends 656 Latest Feb-18 by Shylo (shylo2425) IOS Friends 1873 Latest Feb-16 by Shylo (shylo2425) Friendsġ3793 messages in 1025 discussions PC Friends 2280 Latest Feb-29 by Lisadengel Avatars for sell 36 Latest Mar-2 by Kid (Kidmagnet) Looking for Marcia, Marcia, Marcia 5 4144 messages in 193 discussions Roll Call! 1349 3/31/2024 0 Comments Halion sonic se library download![]() ![]() There are 3 different editions of HALion available: Version 6 of HALion, announced at Winter NAMM Show on 19 January 2017 (alongside version 3 of HALion Sonic), introduced new features such as customization macros and user scripting (using a LUA-based scripting language ). Version 5 (and version 2 of HALion Sonic) followed in July 2013 with new instruments and effects. Version 4 of the main edition of HALion was announced at Musikmesse 2011, introducing sound synthesis features to expand beyond purely sample-based audio. In June 2010, Steinberg announced the HALion Sonic edition of the software, positioned as the successor to their previous Hypersonic 2 software instrument, including both sampling and sound synthesis functionality. In July 2009, Michael Fakesch (former member of the band Funkstörung) produced a score for the Philips commercial Carousel (which won the Film Grand Prix award at the Cannes Lions International Advertising Festival ) using the HALion Symphonic Orchestra and Cubase. On 1 April 2009, Steinberg released the version 3.5 update to HALion, which introduced 64-bit support. Īt Musikmesse 2006, Steinberg announced a specific version of the software, known as the HALion Symphonic Orchestra, which included the samples to represent a full orchestra. By the year 2004, it had reached version 3. The first version of HALion was released by Steinberg in 2001. HALion can be used either as a standalone application, or as a VST, AU, or AAX (Avid Audio eXtension for Pro Tools) plug-in within digital audio workstation software. It can also use multiple forms of sound synthesis to produce other software synthesizer audio. It uses a sample-based approach to emulate the acoustic sounds of a full orchestra, such as the strings, brass, woodwind, and percussion sections, with multiple configurations for each instrument, allowing for variations in timbre. ![]() HALion is a software instrument application, created by German music software company Steinberg for macOS and Windows. en /products /vst /halion _und _halion _sonic /halion. 3/30/2024 0 Comments Xinput test download![]() The results will be added to your test page. If you want to compare the results of different tests, simply repeat the test. You will see the minimum, average, and maximum latency, as well as the polling rate and jitter. When the test is complete, the program will redirect you to the site with the results. This will allow the program to collect data about the gamepad's latency. If your gamepad is not detected, make sure it is connected correctly and try again.Īfter the gamepad is detected, the program will ask you to move the left stick in a circle without stopping. Gamepadla will automatically detect connected gamepads. Just double-click on the fileGamepadla.exe filefile to launch the program. Make sure your operating system recognizes the gamepad. You can connect your gamepad via Bluetooth, with a cable, or with the included receiver. The results obtained from Gamepadla should be used as a guide, not as an exact measurement of input latency. Therefore, although Gamepadla can give a general idea of the "response" of a gamepad, it cannot accurately measure input latency in the traditional sense. ![]() This method of measurement can be affected by various factors, including the quality of the gamepad, the speed of the computer's processor, the speed of event processing in the Pygame library, and so on. Gamepadla measures the delay between successive changes in the position of the analog stick on the gamepad, rather than the traditional input latency, which measures the time between pressing a button on the gamepad and a response in a program or game. Gamepadla works with most popular gamepads and supports DInput and XInput protocols, making it a versatile solution for testing different types of controllers. This tool will help you get accurate data about your controller's performance, which can be useful for gamers, game developers, and enthusiasts. Gamepadla is an easy way to check the polling rate of your gamepad. UPD: In version 3.0.Gamepads polling rate and synthetic latency testerĪ website with a catalog of tested gamepads: The jar file with the java code in should be in maven central, you’ll need the jinput.jar and jinput-natives-all.jar that contains the native binaries. Please leave any suggestions for improvement and comments in the comments section. The code is still under development and may contain bugs. Program can be downloaded from here code is open source, so you can check it or suggest changes. For real latency measurement, it is better to use the following methods. I decided not to add the gamepad latency output functionality, since such tests show only synthetic data, which usually has nothing to do with real latency. But if there are any errors, write to me and I will improve the code. I tested it on many gamepads and compared the results with XInputTest, so far, everything matches. There's no need to wait for anything, just start moving the stick and the result will be displayed immediately. Please don't report or aggressively downvote reposts as we are always getting new subscribers and the content may be new to them.Īn example of testing a Asus Rog Raikiri ControllerĪ simple and effective program for instantly measuring the poling rate of your gamepad.It supports up to 4 controllers to check. Otherwise reported links will be automatically re-approved after the moderators scratch their privates. X Input Tester is a free way to check if your X Input Device is working properly. Message the moderators why you reported a link.This is ONE area where you should feel comfortable contacting and talking to a moderator if you want swift action. Hate speech and bullying will not be tolerated. We ARE an open and affirming subreddit welcoming LBGTA members and people with diverse views on life.That includes downvoting interesting submissions or honest opinions in comments just because YOU have a pet peeve. No doxing! InstaBan and reported to Reddit Admins. SOME Subscriber/Vendor Spam is allowed but make sure it is INTERESTING CONTENT (HINT: We love promos, discounts, and freebies!). test the program : Let us consider the data of example 7.2.2 to test this. SPECIFICALLY no buying or selling or trading. Download this program from file name : integration.m. We don't care if you're all about PC or some console - we're all here for our shared interest in controllers. Try running XInputTest.exe If it runs Good Job, you don't need anything else. Alternitivly you can just you windows built in ZIP unpackager. Posts that don't have flair will be removed. How to setup: Download as a Zip or TAR Open the Zip or TAR with something like Winrar or 7ZIP for TAR AND ZIP. Please use one of those other versions to enjoy the sub to its fullest, thanks! Welcome to /r/controller ! Mods, unboxings, 3rd party - what ever it may be, it probably belongs here! This subreddit is optimized for the reddit redesign and official app, old.reddit does not get updated. 3/30/2024 0 Comments Gang gang cockatoo chibi![]() Don’t remove all disease from your plants as they can be a source of food for cockatoos and other native birds as the acacia galls are for the Gang Gang cockatoos. Plant She-oaks and other native plants they frequent and feed off. There is a lesson to learn from these birds if you want to attract them to your garden. Gang Gang Cockatoos are found in south-eastern parts of Australia from the mid-north coast of NSW south along the coast to the far south-west Victoria. They range in length from 32 to 37 cm, with a wingspan of 62 to 76 cm. Yes, they are big and require quite expensive cages, but also be prepared for lots of noises. They are beautiful but not the best choice for a pet. ![]() They researched the best location to see each of the fourteen species and planned the trip from. Earlier this year he asked his students to plan an expedition to photograph every species of cockatoo in Australia. They are primarily slate-grey, with the males easily identified by their scarlet head and wispy crest, while females have a grey head and crest and feathers edged with salmon pink on the underbelly. Major Mitchell’s cockatoo reaches 20-24 inches and weighs over 2 pounds. DARREN HAMLEY is a coordinator of gifted education at Willetton Senior High School in Western Australia. In the wild they feed on seeds as well as insects, including the pupa or maggot in acacia galls. Gang -gang Cockatoos are one of the more distinctive and charismatic Australias birds. The male birds have a light red head and crest, hence their other name, Red-crowned Cockatoo or Helmeted Cockatoo. They are owl-like small (33-36cm ) dark grey cockatoos patterned by pale margins to rather square feathers with an olive wash on the wings. The Gang Gang Cockatoo (Callocephalon fimbriatum) can be difficult to keep in captivity because of feeding and feather-pulling problems. They only have one baby which stays with the parents for several months learning from them until it is fully mature. They breed between March and August and nest in a layer of woodchips in large tree hollows, often high off the ground. They have also been known to learn how to undo the latches of their cages, exhibiting their great intelligence. Both sexes incubate the 2-3 eggs for about a month and care for the young. They are notoriously persistent shriekers and don’t take no for an answer. The female chooses a nest hollow in a suitable tree and both sexes prepare the nest for egg-laying, lining it with wood-chips and dust by chewing at the sides of the hollow. They are unobtrusive but large (46-51cm ) blackish brown birds and are found in coastal forest and open inland woodland in eastern Australia. Gang-gang Cockatoos form close, monogamous pairs. An uncommon sight in the eastern states of Australia is the Glossy Black-Cockatoo (Calyptorhynchus lathami) also known as the Casuarina Cockatoo as it feeds on She-oak (Casuarina) cones. Recommended volume per well for 1.0 mm mini gelġ0 μL for electrophoresis, 5 μL for blottingĥ μL for electrophoresis, 2. ~160, 120, 80, 60, 50, 40, 30, 20, 15, 10 kDaĮach protein standard contains a 6X His-tag enabling the detection with the InVision His-tag In-gel StainĬolorimetric or methods that detect phosphorylated proteins such as Pro-Q Diamond phosphoprotein gel stainsĬolorimetric or methods that detect glycosylated proteins such as Pro-Q Glycoprotein stain kits Peppermint Stick Phosphoprotein Molecular Weight StandardsĬand圜ane Glycoprotein Molecular Weight Standards Using a prestained protein ladder when running a western blot helps when you need to: - monitor protein separation during SDS-polyacrylamide gel electrophoresis. Tagged- proteins in the 10 -250 kDa range contain a Strep-tag™ II sequence and can be detected on western blots using Strep-Tactin™ conjugates or an antibody against Strep-tag™ II sequenceĭirect visualization can be achieved through standard staining protocols (e.g., Coomassie, etc.) Tagged-proteins contain an integral Strep-tag™ II sequence and can be detected on western blots using Strep-Tactin™ conjugates or an antibody against Strep-tag™ II sequence PageRuler Unstained Broad Range Protein LadderĪccurate estimation across a broader range Recommended Volume per well for 1.0 mm gel (μL)Ĭolorimetric, NIR fluorescence (700nm channel, blue bands), RGB fluorescence (550 nm channel, orange bands) Visible monitoring of gel separation and transfer efficiency PageRuler unstained protein ladders (Cat. We offer a comprehensive suite of solutions for every step of the western blotting. For most researchers, we generally recommend:Ī) ab116027 for proteins between 10 and 180 kDaī) ab116028 for larger proteins up to 245 kDaĬ) ab116029 for smaller proteins down to 3.5 kDaĭ) ab116029 or ab234592 for studying smaller and larger proteins at the same time.Spectra Multicolor Broad Range Protein Ladderīest multicolor prestained ladder for routine applicationsĤ colors for improved visualization during separation and transferĪnalysis of high molecular weight proteins Western blotting is an extremely useful tool for. When choosing the right pre-stained protein ladder, the most important choice is based on the size of your protein of interest. Prestained ladders also can use highlight bands of a different color, to make it easier to identify different size bands. Protein ladders are most convenient to use when they are supplied ready to use, with no heating, dilution, or addition of reducing agent required before loading onto the gel. Three different chromophores are bound to the proteins, producing a brightly colored ladder. Unstained protein ladders are more accurate for sizing proteins, as the dyes used in prestained ladders can slightly distort the apparent size of the protein ladder proteins on the gel. The Spectra Multicolor High Range Protein Ladder is a mixture of eight (8) blue-, green-and orange-stained proteins (40 to 300 kDa) for use as a high-MW standard in gel electrophoresis and western blotting. However, an unstained protein ladder can only be visualized following staining with Coomassie or a similar non-specific protein stain. Using an unstained protein ladder is very useful when you need to accurately calculate the size of your protein. Western blot protein ladders are designed for protein molecular weight estimation directly on the blot during detection. calculate the approximate size of your protein by overlaying an image of the membrane with the ladder with the image generated by antibody staining. verify transfer efficiency between gel and membrane (PVDF, nylon, or nitrocellulose) monitor protein separation during SDS-polyacrylamide gel electrophoresis Using a prestained protein ladder when running a western blot helps when you need to: |
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